Question 1:

Which DNA sequencing technique uses the synthesis of DNA strands to determine the sequence?

Explanation: A) Sanger sequencing is a DNA sequencing technique that uses the synthesis of DNA strands and chain termination to determine the sequence.

Question 2:

Which DNA sequencing technique allows for massively parallel sequencing of millions of DNA fragments?

Explanation: C) Next-generation sequencing (NGS) allows for massively parallel sequencing of millions of DNA fragments, enabling high-throughput sequencing.

Question 3:

Which DNA sequencing technique uses fluorescently labeled nucleotides and detects their incorporation?

Explanation: A) Sanger sequencing uses fluorescently labeled nucleotides and detects their incorporation to determine the DNA sequence.

Question 4:

Which DNA sequencing technique relies on the amplification of DNA using specific primers?

Explanation: B) Polymerase chain reaction (PCR) is a technique that relies on the amplification of DNA using specific primers, which can be used in DNA sequencing.

Question 5:

Which DNA sequencing technique utilizes the CRISPR-Cas9 system to target specific DNA sequences?

Explanation: D) CRISPR-Cas9 is a gene editing system that can be used for targeted DNA sequencing by utilizing the Cas9 protein to target specific DNA sequences.