Question 1:

Which of the following techniques is commonly used to amplify specific DNA sequences?

Explanation: PCR (Polymerase Chain Reaction) is a widely used technique that allows for the amplification of specific DNA sequences in a laboratory setting.

Question 2:

Which enzyme is responsible for synthesizing a complementary DNA strand during PCR?

Explanation: DNA polymerase is the enzyme responsible for synthesizing a complementary DNA strand during PCR, using the original DNA strand as a template.

Question 3:

Which of the following is an essential component of a plasmid vector used in gene cloning?

Explanation: The origin of replication is an essential component of a plasmid vector used in gene cloning, as it allows the plasmid to replicate within the host organism.

Question 4:

What is the purpose of gel electrophoresis in gene cloning?

Explanation: Gel electrophoresis is used to separate DNA fragments based on their size, allowing researchers to analyze and isolate specific fragments of interest during gene cloning.

Question 5:

Which technique is commonly used to introduce foreign DNA into host cells during gene cloning?

Explanation: Transformation is a commonly used technique in gene cloning to introduce foreign DNA into host cells, such as bacterial cells, allowing them to take up and replicate the DNA of interest.