Question 1:

What is the purpose of recombinant DNA technology?

Explanation: The purpose of recombinant DNA technology is to transfer genetic material between different organisms. This allows scientists to introduce specific genes into organisms, creating genetically modified organisms (GMOs) or producing useful products through genetic engineering.

Question 2:

Which enzyme is commonly used to cut DNA molecules at specific sequences during recombinant DNA technology?

Explanation: Restriction enzymes are commonly used to cut DNA molecules at specific sequences during recombinant DNA technology. These enzymes recognize specific DNA sequences and cleave the DNA at those sites, allowing for the manipulation and recombination of DNA fragments.

Question 3:

What is a plasmid in the context of recombinant DNA technology?

Explanation: In the context of recombinant DNA technology, a plasmid refers to a small, circular DNA molecule found in bacteria. Plasmids are often used as vectors to carry and replicate foreign DNA fragments in host cells during genetic engineering experiments.

Question 4:

What is the purpose of a selectable marker in recombinant DNA technology?

Explanation: The purpose of a selectable marker in recombinant DNA technology is to identify transformed cells that have taken up foreign DNA. Selectable markers, such as antibiotic resistance genes, allow researchers to selectively grow and isolate cells that have successfully incorporated the desired genetic material.

Question 5:

What is the purpose of DNA ligase in recombinant DNA technology?

Explanation: DNA ligase is used to join DNA fragments together in recombinant DNA technology. It catalyzes the formation of phosphodiester bonds between the sugar-phosphate backbones of DNA molecules, allowing the fragments to be ligated and the genetic material to be recombined.