5 Q&A: Agarose Gel Electrophoresis: Step-by-Step Protocol and Applications

Question 1:

What is the purpose of agarose gel electrophoresis?

Question 2:

What is the purpose of adding ethidium bromide to the agarose gel during electrophoresis?

Question 3:

Which type of charge does DNA or RNA carry in agarose gel electrophoresis?

Question 4:

Which buffer is commonly used for agarose gel electrophoresis of nucleic acids?

Question 5:

Which agarose gel concentration is generally used for resolving larger DNA fragments?

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