Question 1:
What is the principle behind chromatography?
Explanation: The correct answer is C) Partitioning of components between stationary and mobile phases. The principle behind chromatography is the partitioning of components between a stationary phase (e.g., solid support) and a mobile phase (e.g., liquid or gas). The components in the sample interact differently with the stationary and mobile phases, leading to their separation.
Question 2:
Which of the following chromatographic techniques is commonly used for separating mixtures of compounds with different polarities?
Explanation: The correct answer is B) Liquid chromatography. Liquid chromatography is commonly used for separating mixtures of compounds with different polarities. In this technique, the stationary phase is a solid support or a liquid immobilized on a solid support, and the mobile phase is a liquid solvent. Compounds in the mixture interact differently with the stationary phase, leading to their separation.
Question 3:
Which chromatographic technique utilizes a solid stationary phase and a mobile phase consisting of a gas?
Explanation: The correct answer is A) Gas chromatography. Gas chromatography utilizes a solid stationary phase (often coated on a column) and a mobile phase consisting of a gas. Compounds in the mixture are vaporized and carried by the gas through the column. The separation is based on the differential interaction of compounds with the stationary phase.
Question 4:
Which type of chromatography relies on the size exclusion principle to separate molecules based on their molecular size?
Explanation: The correct answer is D) Size exclusion chromatography. Size exclusion chromatography (SEC), also known as gel filtration chromatography, separates molecules based on their molecular size. In this technique, a porous stationary phase allows smaller molecules to enter the pores and travel a longer path, while larger molecules are excluded and travel a shorter path, resulting in their separation.
Question 5:
Which chromatographic technique is commonly used for the separation of charged molecules based on their affinity for a charged stationary phase?
Explanation: The correct answer is C) Ion exchange chromatography. Ion exchange chromatography separates charged molecules based on their affinity for a charged stationary phase. The stationary phase is typically a resin containing charged groups (e.g., cation or anion exchange resin). The separation is achieved by varying the ionic strength or pH of the mobile phase to promote binding or elution of the analytes.
- Click to Check the published quizzes on various Categories
- Click to Practice various fundamentals of PMP Certification
- Click to Check Published courses on Piping Engineering
- Click to check Video Liberary (with more than 600+ Videos)