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Question 1:

What is the basic principle of spectroscopy?

A) Absorption of light by a sample
B) Emission of light by a sample
C) Scattering of light by a sample
D) Interference of light waves

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Explanation: The correct answer is A) Absorption of light by a sample. The basic principle of spectroscopy is the measurement of the interaction between light and matter, particularly the absorption of light by a sample. This interaction provides valuable information about the composition, structure, and properties of the sample.

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Question 2:

Which property of light is used in spectroscopy to obtain information about the sample?

A) Intensity
B) Wavelength
C) Frequency
D) Polarization

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Explanation: The correct answer is B) Wavelength. Spectroscopy utilizes the property of light wavelength to obtain information about the sample. The interaction of light with the sample leads to changes in the wavelength, providing insights into the sample's molecular structure, energy levels, and chemical composition.

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Question 3:

Which of the following techniques is based on the principle of spectroscopic analysis?

A) Chromatography
B) Mass spectrometry
C) Electron microscopy
D) Spectrophotometry

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Explanation: The correct answer is D) Spectrophotometry. Spectrophotometry is a technique based on the principle of spectroscopic analysis. It involves measuring the intensity of light before and after interaction with a sample, providing information about the concentration and absorption characteristics of the sample.

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Question 4:

What does the spectroscopic analysis of emission spectra reveal?

A) Energy levels of the sample
B) Absorption characteristics of the sample
C) Scattering properties of the sample
D) Polarization behavior of the sample

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Explanation: The correct answer is A) Energy levels of the sample. The spectroscopic analysis of emission spectra reveals the energy levels of the sample. When excited, the sample emits light at specific wavelengths corresponding to the energy differences between its energy levels. By analyzing these emission wavelengths, valuable information about the sample's energy states can be obtained.

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Question 5:

What is the purpose of a reference spectrum in spectroscopic analysis?

A) To determine the concentration of the sample
B) To calibrate the spectrophotometer
C) To identify the presence of impurities
D) To account for instrumental effects

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Explanation: The correct answer is D) To account for instrumental effects. A reference spectrum is used in spectroscopic analysis to account for instrumental effects, such as variations in light intensity, wavelength accuracy, or system response. By comparing the sample spectrum with the reference spectrum, the influence of these instrumental factors can be minimized, allowing more accurate analysis of the sample.