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Question 1:

What is the purpose of differential centrifugation?

A) Separating molecules based on size
B) Separating molecules based on density
C) Separating molecules based on charge
D) Separating molecules based on solubility

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Explanation: The correct answer is A) Separating molecules based on size. The purpose of differential centrifugation is to separate molecules or particles based on their size or sedimentation rates under centrifugal force.

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Question 2:

Which factor is primarily utilized in differential centrifugation to separate molecules?

A) Density
B) Viscosity
C) Charge
D) Size

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Explanation: The correct answer is D) Size. Differential centrifugation primarily utilizes the size of molecules or particles to separate them. Larger particles sediment faster and separate from smaller particles during centrifugation.

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Question 3:

What is the general process of differential centrifugation?

A) Creating a density gradient
B) Centrifuging at a fixed speed
C) Repeating centrifugation cycles
D) Sequentially varying the centrifugal force

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Explanation: The correct answer is D) Sequentially varying the centrifugal force. The general process of differential centrifugation involves subjecting the sample to multiple centrifugation steps at different speeds or forces. This sequential variation in centrifugal force allows the separation of particles based on size.

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Question 4:

What is the pellet obtained in differential centrifugation called?

A) Supernatant
B) Residue
C) Filtrate
D) Solvent

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Explanation: The correct answer is B) Residue. The pellet obtained after centrifugation in differential centrifugation is called the residue. It contains the larger particles or molecules that have sedimented to the bottom of the centrifuge tube.

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Question 5:

What is the purpose of increasing the centrifugal force in differential centrifugation?

A) To separate smaller particles
B) To separate larger particles
C) To separate soluble molecules
D) To increase the efficiency of separation

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Explanation: The correct answer is D) To increase the efficiency of separation. Increasing the centrifugal force in differential centrifugation helps to separate smaller particles or molecules. By applying higher centrifugal forces, the sedimentation rate of smaller particles increases, improving the separation efficiency.