UV-Visible spectrometry is a technique used to measure the absorption or transmission of which type of electromagnetic radiation?
Explanation: The correct answer is A) Ultraviolet (UV) radiation. UV-Visible spectrometry involves the measurement of absorption or transmission of electromagnetic radiation in the UV and visible regions of the spectrum, which includes wavelengths ranging from about 190 to 800 nanometers (nm). This technique is widely used in various fields, including chemistry, biochemistry, and materials science.
What is the principle behind UV-Visible spectrometry?
Explanation: The correct answer is C) Absorption of light. UV-Visible spectrometry is based on the principle that molecules absorb specific wavelengths of light as a result of electronic transitions within their structure. By measuring the extent of this absorption, information about the sample's chemical composition, concentration, and other properties can be obtained.
Which of the following is commonly used as a reference standard in UV-Visible spectrometry?
Explanation: The correct answer is D) Caffeine (C8H10N4O2). Caffeine is commonly used as a reference standard in UV-Visible spectrometry. It has a well-defined absorption spectrum in the UV region, making it suitable for calibration and verification of spectrophotometers and the measurement of unknown samples.
Which of the following statements is true about UV-Visible spectrometry?
Explanation: The correct answer is D) It provides information about the electronic structure of a sample. UV-Visible spectrometry provides valuable information about the electronic structure of a sample by measuring the absorption or transmission of light. This technique allows researchers to determine the presence and concentration of specific compounds, assess chemical reactions, and investigate the behavior of molecules in various environments.
What is the typical unit of measurement used for absorbance in UV-Visible spectrometry?
Explanation: The correct answer is D) Absorbance (AU). Absorbance, also known as optical density, is the typical unit of measurement used in UV-Visible spectrometry. It quantifies the amount of light absorbed by a sample at a specific wavelength and is calculated using the Beer-Lambert Law. The absorbance values are dimensionless and usually represented in arbitrary units (AU) or absorbance units.